2 edition of Hypoxia Responsive Vectors Targeting Astrocytes in Glioma found in the catalog.
by INTECH Open Access Publisher
Written in English
|Contributions||Howard M. Prentice, author, Janet C. Blanks, author|
|The Physical Object|
|Pagination||1 online resource|
Hypoxia-responsive ionizable liposome delivery siRNA for glioma therapy Article (PDF Available) in International Journal of Nanomedicine Volume February with Reads. A Hypoxia-Responsive Glial Cell–Specific Gene Therapy Vector for Targeting Retinal Neovascularization Manas R. Biswal, 1 Howard M. Prentice, 2, 3 C. Kathleen Dorey, 4 and Janet C. Blanks 2 1 Integrative Biology PhD Program, Florida Atlantic University, Boca Cited by:
Engineered lentiviral vector targeting astrocytes In vivo thus improving the selective targeting of lentiviral vectors to astrocytes. The shRNA targeting the mouse Cx43 (passenger-loop-guide. A hypoxia-responsive glial cell–specific gene therapy vector for targeting retinal neovascularization.
Hypoxia-inducible factor 1 (HIF-1) is one of the master regulators that orchestrate the cellular responses to hypoxia. It is a heterodimeric transcription factor composed of α and β subunits. The α subunit is stable in hypoxic conditions but is rapidly degraded in by: Glioblastoma is the most common brain tumor in adults. Advanced glioblastomas normally contain hypoxic areas. The primary cellular responses to hypoxia Cited by:
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Hypoxia Responsive Vectors Targeting Astrocytes in Glioma regarded to be the major factor for development of new vessels both in physiological and pathological angiogenesis. Astrocytes have the ability to adjust cerebral blood flow to maintain constant PO 2 and PCO 2 of the brain parenchyma.
Release of ATP in the brainstem, presumably by local astrocytes, helps to maintain breathing and counteract hypoxia-induced depression of the respiratory by: neovascularization, a new hypoxia-responsive, glial-specific AAV vector was constructed to target Müller cells, and its specificity and responsiveness were verified in.
For instance, monoamine oxidase B (MAO‐B) which is a target for the antidepressant deprenil and is localized almost exclusively in astrocytes (Riederer et al., ), has recently attracted attention because it can be used as an activator for pro‐drugs that, after the reaction with MAO‐B, become cytotoxic for glioma cells, which typically Cited by: The ability of tumor cells to adapt to hypoxia and survive is regulated by a family of transcription factors called hypoxia inducible factors (HIFs), HIFs are the central transcription factors which regulate the expression of a large number of hypoxia-responsive genes, the expressions of more than 40 target genes, whose protein products are implicated in angiogenesis, metabolism, and cell Cited by: Primary astrocytes that were exposed to 3-hours hypoxia with no reoxygenation showed the largest redistribution to smaller sized mitochondria compared to the 3-hours hypoxia then hours reoxygenation and normoxia groups (Fig.
1C).In the normoxia group, % of mitochondria fell within the – μm 2 range. After 3-hours of hypoxia these values increase to % (p = ) Cited by: 2.
Upon entering the tumor site and being subjected to the hypoxic tumor environment, the PEG groups are detached from the PAMAM surface because AZO, the hypoxia-responsive bioreductive linker, could be broken in a reductive microenvironment and reduced to aminoaromatics, resulting in the exposure of the small size and positive charge of PAMAM, which facilitates the penetration of by: 5.
Engineered lentiviral vector targeting astrocytes In vivo. are highly neurotropic. We used mokola pseudotyping to shift the tropism of lentiviral vectors toward astrocytes and a detargeting strategy with miRNA to eliminate residual expression in neuronal cells.
we showed that incorporating target sequences for the neuron‐specific Cited by: The exposure of U87, SNB75 and U cells to hypoxia (1% O 2) for 72 h resulted in a marked difference in their morphology compared to normoxia (20% O 2) cultured cells particularly in U87 and SNBUnder hypoxic conditions the cells had a more elongated morphology and were more loosely clustered than normoxia cultured cells ().The migration/invasion potential of U87 cells was tested using Cited by: Glioblastoma multiforme is the most common and the most aggressive primary brain tumor.
It is characterized by a high degree of hypoxia and also by Cited by: In the present study we addressed the question whether GAPDH expression is regulated by hypoxia in human glioblastoma cells in vitro and in human glioma tumor samples.
The answer of this question will provide insight into practical applications of GAPDH as an internal standard in investigations of hypoxia-inducible gene expression or as a target for tumor therapeutic approaches in human by: The role of hypoxia-responsive molecules, especially hypoxia-inducible factor-1 (HIF-1), in glioma tumorigenesis is explored.
Treatment modalities regulated by hypoxia are proposed and some potential strategies reviewed. The pro-gression of a low-grade astrocytoma to a glioblastoma multiforme may be mediated by hypoxia-induced phenotypic.
In view of the recent finding that hypoxia plays a key role in the directed migration of NSCs towards tumors, hypoxia-targeting approach can be employed to regulate transgene expression in NSC vectors. Hypoxia is a hallmark of the tumor microenvironment. In most human tissues, the physiological oxygen tensions range from 2% to 9%.Cited by: Extensive hypoxic regions are the daunting hallmark of glioblastoma, as they host aggressive stem-like cells, hinder drug delivery and shield cancer cells from the effects of radiotherapy.
Nanotechnology could address most of these issues, as it employs nanoparticles (NPs) carrying drugs that selectively accumulate and achieve controlled drug release in tumor by: 9. Conclusion: circDENND2A is required for the hypoxia-induced malignancy of glioma cells and functions by sponging miRp.
Keywords: circDENND2A, miRp, Glioma, Hypoxia, Migration, Invasion Introduction Glioma is the most prevalent and malignant tumor of the central nervous system, ori-ginating from glial cells .Cited by: Glioma stem-like cells (GSCs) have been identified as a subpopulation of tumor cells that are thought to be largely responsible for resistance to treatment.
Intratumoral hypoxia contributes to maintenance of the GSCs by supporting the critical stem cell traits of Cited by: to target and manipulate astrocytes. In Table 1, we have summarized some of the major viral strategies to target astrocytes in specific brain regions.
We briefly review these viral vectors below: Lentiviral targeting of astrocytes LV along with AAV, are the most commonly used viral vectors used to facilitate targeted gene expression in.
Hypoxia is negatively associated with glioblastoma (GBM) patient survival and contributes to tumour resistance. Anti-angiogenic therapy in GBM further increases hypoxia and activates survival Cited by: To achieve glioma-specific expression, glioma-specific vectors are generally composed of promoters, enhancers, and/or 5′ UTR that are responsive to glioma-specific transcription factors.
GFAP is a kDa intracytoplasmic filamentous protein that constitutes the cytoskeleton of the by: Hypoxia Potently Induced HIF2α Protein Expression in Glioma Stem Cells (A–G) Glioblastoma stem and non-stem cells isolated from multiple samples were treated with DFX to mimic hypoxia for the indicated times.
Nuclear and cytoplasmic fractions (B) or total cell lysate (A, C, and E–G) were by:. Human pluripotent stem cell-derived neural precursor cells (hNPCs) require long-term culturing to differentiate into astrocytes, retarding functional studies of human astrocytes.
Nakashima and colleagues have developed a method for rapid induction of astrocytes from hNPCs cultured at low oxygen levels. Hypoxia induces epigenetic change, allowing hNPCs to differentiate into by: 8. One of the main early cellular events evoked upon exposure to hypoxia is activation of HIF-1, a key heterodimeric transcription factor.
In reduced oxygen conditions, HIF-1 binds to hypoxia-responsive elements (HREs) and induces transcription of various target genes involved in tumor angiogenesis, invasion, cell survival, and glucose by: Moreover, the additional incorporation of hypoxia-responsive promoters to trigger the gene therapy would exploit the unique physiology of many tumors, while targeting Cited by: