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3 edition of The role of phosphodiesterase and cAMP during D.Discoideum aggregation found in the catalog.

The role of phosphodiesterase and cAMP during D.Discoideum aggregation

James Grutsch

The role of phosphodiesterase and cAMP during D.Discoideum aggregation

by James Grutsch

  • 128 Want to read
  • 7 Currently reading

Published .
Written in English


Classifications
LC ClassificationsMicrofilm 69101
The Physical Object
FormatMicroform
Paginationiv, 84 l.
Number of Pages84
ID Numbers
Open LibraryOL1368287M
LC Control Number92895583

Upon starvation, amoebae of the mutant strain HPX are unable to aggregate. Previous work has shown that this aggregateless character was associated with a nearly complete block in the production of the phosphodiesterase by these cells. Aggregation of the HPX amoebae can be induced with exogenous phosphodiesterase. In the present work, we show that both the aggregateless character . A phosphodiesterase (PDE) is an enzyme that breaks a phosphodiester y, phosphodiesterase refers to cyclic nucleotide phosphodiesterases, which have great clinical significance and are described below. However, there are many other families of phosphodiesterases, including phospholipases C and D, autotaxin, sphingomyelin phosphodiesterase, DNases, RNases, .

systems of development. cAMP-S can als boe used to investigate the role of chemo-taxis in cell aggregation At high., uniform cAMP-S concentration is s chemotaxis suppressed, so that aggregation ca bne studied in its absence. MATERIALS AND METHODS Strains. The D. discoideum strains used wer NC-4e, Ax-2, an d clones Mi and M2 of strain v ase, deum has an intracellular PDE specific for cGMP [8, This enzyme is activated 4- fold by concentrations of cGMP within the range A group of chemotactic mutants of deum (stmF ‘streamer’ mutants) have been isolated which show an alteration in the cGMP response to CAMP [12].

Start studying Phosphodiesterase inhibitors. Learn vocabulary, terms, and more with flashcards, games, and other study tools. cGMP speciific phosphodiesterases and are utilized for smooth muscle relaxation and inhibition of platelet aggregation, as well as pulmonary hypertension (adenosine acts as a vasodilator and plays a role in. promoters are also hallmarks of the PdsA and ACA genes in D. discoideum (Faure et al., ; Galardi-Castilla et al., ), suggesting that the novel role of cAMP signalling during aggregation of group 4 species was achieved by distal insertion of new cis-regulatory regions in existing cAMP signalling genes.


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The role of phosphodiesterase and cAMP during D.Discoideum aggregation by James Grutsch Download PDF EPUB FB2

Over-accumulation of cAMP in the extracellular medium since aggregation ca in be induced the absence of added phosphodiesterase under conditions facilitating diffusion of the nucleotide.

This suggests that phosphodiesterase is no it involven the generatiod n or recognition of cAMPCited by: cAMP acts as a primary signal and is regulated by a secreted cyclic nucleotide phosphodiesterase (PDE) throughout development in Dictyostelium discoideum.

Expression of the PDE gene (pde) is controlled by promoters specific to vegetative growth (prV), aggregation (prA), or late development (prL).Cited by:   The cyclic nucleotides cAMP and cGMP play a central role in diverse signal transduction processes in Dictyostelium discoideum. cAMP mediates chemotaxis during cell aggregation, and controls gene expression during development.

cGMP regulates cytoskeletal organization affecting shape, stability and motility of single cells. The intracellular concentration of cAMP and cGMP is determined Cited by:   cAMP plays an essential role duringDictyostelium development both outside and inside the cell.

Membrane-bound receptors and adenylyl cyclase are responsible for sensing and producing extracellular cAMP, whereas a phosphodiesterase is responsible for maintaining a low basal level.

The molecular events underlying this type of hormone like signalling, which are now beginning to be Cited by: The cyclic nucleotide phosphodiesterase (phosphodiesterase) plays essential roles throughout the development of Dictyostelium discoideum. It is crucial to cellular aggregation Cited by: Abstract.

For many years it has been known that developing cells of Dictyostelium discoideum show periodic surges as they aggregate. When it was discovered that the cells were responding chemotactically to cAMP gradients produced within the populations, experiments were carried out that demonstrated similar periodic changes in the concentration of extracellular cAMP.

The development and cellular differentiation of Dictyostelium discoideum are disrupted in transformants secreting high levels of the cyclic nucleotide phosphodiesterase. The aggregation of these cells in the early stage of development proceeds rapidly and without the.

Disruption of Dictyostelium discoideum morphogenesis by overproduction of cAMP phosphodiesterase. and demonstrate the role of cAMP during the aggregation phase of development as well as in the. Cyclic AMP (cAMP) appears to play multiple roles in the development of the social ameba Dictyostelium discoideum, serving as the chemoattractant mediating aggregation, and perhaps also regulating gene transcription in both early and late stages of differentiation.

Progress in understanding the mechanism of activation of the adenylate cyclase in D. discoideum has been frustrated by the. Changes in intracellular and extracellular cAMP levels are reported for the cellular slime mold Dictyostelium discoideum during its development on filter supports.

Examined were axenically and bacterially grown strain A3 and bacterially grown NC In each case a major peak in cAMP occurred during aggregation. change the wave generati on processes of D. discoideum, including advective flows [ 6 ], e x ternal magnetic forces during the early stage of D.

discoideum morphogenesis [ 7 ], just to name a few. Characterization of a cAMP-stimulated cAMP Phosphodiesterase inDictyostelium discoideum Article (PDF Available) in Journal of Biological Chemistry (16) May with 16 Reads. The ability of N6-(aminohexyl)-adenosine-3′:5′-monophosphate to mimic the actions of cAMP on cell differentiation and chemotaxis was investigated.

Thi. Abstract. A cyclic nucleotide phosphodiesterase, PdeE, that harbors two cyclic nucleotide binding motifs and a binuclear Zn 2+-binding domain was characterized other eukaryotes, theDictyostelium domain shows greatest homology to the kDa subunit of the pre-mRNA cleavage and polyadenylation specificity factor.

The Dictyostelium PdeE gene is expressed at its highest levels. This aggregation process shares similarities to pre-sorocarpic aggregation in D. discoideum, including cAMP pulses and cell streaming, but on a much smaller scale, as only~ cells contribute to.

Another important function of cAMP is its role as chemoattractant, coordinating both aggregation and fruiting body formation in D. discoideum, while secreted cAMP has additional roles in the induction of aggregation genes and prespore genes and the repression of stalk genes (Figure 2).

A phosphodiesterase is also excreted by aggregating cells6,7 activity of which modulates the cyclic AMP gradient. Thus, a general model for communication between cells during aggregation is available. Pharmacological characterization of cyclic and cAMPappear to play a role in gene expression in D.

discoideum (, 18, 26, 29, 41). aggregation competent and acquired cAMP receptors. The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum contains three promoters specific for growth, aggregation, and late development.

M Faure Department of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, New York Dipyridamole (Persantine) is a pyrimidopyrimidine derivative that inhibits the enzyme phosphodiesterase in platelets, thus increasing intraplatelet levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which eventually inhibit platelet aggregation.

The increased cAMP and cGMP inside platelets also potentiate platelet inhibitory effect of. Abstract. In Dictyostelium discoideum extracellular cyclic AMP (cAMP), as shown by previous studies, induces a transient accumulation of intracellular cyclic guanosine-5'-monophosphate (cGMP), which peaks at 10 s and recovers basal levels at 30 s after stimulation, even with persistent cAMP stimulation.Phosphodiesterase inhibitors work by inhibiting PDE3 an enzyme that breaks down cAMP cAMP accumulates within cardiac cells & increases contractility - positive inotropic effect Increased contractility, increases the force of contrction, also increasing cardiac output.Europe PMC is an archive of life sciences journal literature.

Abstract. The cyclic nucleotide phosphodiesterase (phosphodiesterase) gene plays essential roles in the development of Dictyostelium discoideum during cellular aggregation and postaggregation morphogenesis.